Journal: bioRxiv

Article Title: Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula x Populus tremuloides

doi: 10.1101/2022.06.08.495362

Figure Lengend Snippet: Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated Cy5 (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. Fluorescence intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.

Article Snippet: We indirectly quantified pectin epitopes by measuring the fluorescence probe (Cy5) intensity (mean number of pixels per unit area) conjugated with the respective primary antibody using the ‘curve spline’ function of the Zeiss ZEN blue software.

Techniques: Control, Fluorescence

Journal: Heliyon

Article Title: Advancements in cyclodextrin-based controlled drug delivery: Insights into pharmacokinetic and pharmacodynamic profiles

doi: 10.1016/j.heliyon.2024.e39917

Figure Lengend Snippet: The types of nanosystem CD used & polymers [ 457 ].

Article Snippet: Diclofenac , β-CD , Cationic polymer with fluorescent probe nanocarrier , [ , ] .

Techniques: Polymer, Modification